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Whole-life Thinking and Engineering the Future

Roger Flanagan

《工程管理前沿（英文）》 2014年第1卷第3期页码 290-296 doi: 10.15302/J-FEM-2014040

摘要： Whole-life thinking for engineers working on the built environment has become more important in a fast changing world. Whole-life thinking is not new, every project attempts to balance the initial capital cost with the operating and maintenance cost of an asset. Engineers are increasingly concerned with complex systems, in which the parts interact with each other and with the outside world in many ways — the relationships between the parts determine how the system behaves. Systems thinking provides one approach to developing a more robust whole-life approach. Systems thinking is a process of understanding how things influence one another within a wider perspective. Complexity, chaos, and risk are endemic in all major projects. New approaches are needed to produce more reliable whole-life predictions. Best value, rather than lowest cost, can be achieved by using whole-life appraisal as a part of the design and delivery strategy.

关键词： whole-life thinking systems thinking complexity chaos risk management through life

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The Research on Subject Behavioral Risk of Whole Life-cycle Water Conservation Projects

Hong-yong Liu,Ji Guo,Wei-tao He

《工程管理前沿（英文）》 2014年第1卷第4期页码 348-352 doi: 10.15302/J-FEM-2014048

摘要： In 2011, water conservation projects construction in China entered a stage of rapid growth. In February 2013 three dam safety accidents occurred due to improper participant subject behaviors. Improving security and risk management of water conservation projects is therefore considered as imminent. It is urgent to research the risks of the participant subject behaviors. This article takes each participating subject in the whole life-cycle of water conservation projects as the research object, analyzes the risk behaviors and risk evolution processes, dissects the cause of the risks of the participating subject behaviors on the basis of the theory of risk effect, and puts forward that reinforcing the penalties, improving criminal cost and strengthening the engineering ethics education is an effective way to solve safety problems of the current projects.

关键词： water conservation projects subject behavior safety accident risk effect engineering ethics

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General framework for bridge life cycle design

Junhai MA, Airong CHEN, Jun HE

《结构与土木工程前沿（英文）》 2009年第3卷第1期页码 50-56 doi: 10.1007/s11709-009-0004-7

摘要： Based on a detailed illustration for bridge life cycle design which comprises the processes of service life design, aesthetics design, performance design, environmental and ecological design, inspection, maintenance and repair design as well as cost analysis, this paper presented a general framework for bridge life cycle design comprising three design phases and six design processes.

关键词： bridge whole life design design phase design process general framework

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可持续发展工程结构全寿命周期

金伟良,钟小平

《中国工程科学》 2012年第14卷第3期页码 100-107

摘要：

以结构全寿命周期为研究时域，运用结构全寿命设计的新理念，构架了工程结构全寿命设计的目标体系，并从目标体系出发，结合工程结构的特点，分析了设计中结构性能、成本、使用寿命的内涵及相互关系。在综合众多影响因素的基础上构建了工程结构全寿命周期设计理论的研究框架，确定了工程结构性能指标、经济指标及时间指标在全寿命设计理论体系中的核心地位，提出了基于性能设计的耐久性极限状态设计新理念，并分析了性能极限状态所具有的特点。

关键词：基于可持续发展的设计理论结构全寿命周期结构可靠性结构寿命周期成本结构使用寿命

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论创造性思维

冯国瑞

《中国工程科学》 2004年第6卷第6期页码 15-21

摘要：

文章首先阐明了创新与创造的联系与区别，然后重点论述了创造性思维的界定、创造性思维的运演机制、创造性思维的重大意义以及全面提高思维主体综合素质等问题。

关键词：创新创造创造性思维思维主体思维客体

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钱学森——科技界的一面旗帜

涂元季

《中国工程科学》 2002年第4卷第2期页码 1-7

摘要：

文章回顾了钱学森的生平，介绍了他得益于良好的家庭教育和学校教育所形成的优秀品格，展示了钱学森在美国师从冯·卡门以后的出众才华和取得的丰硕成就；文章以鲜为人知的大量事实描述了钱学森为争取返回祖国而进行的不屈不挠的斗争，以及他回到祖国后为了我国的航天事业呕心沥血的事迹，并以具体事例向人们揭示了钱学森对待金钱、荣誉和地位的崇高思想境界与高尚品德。

关键词：生平求知科技攻关工作作风思想品德革命情操

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Organizational evolution of project management teams over the whole lifecycle of megaprojects: Case study

《工程管理前沿（英文）》页码 439-454 doi: 10.1007/s42524-022-0211-7

摘要： Project management teams are critical in the implementation of megaprojects, but their evolution throughout the project lifecycle has not been clearly explained. This paper explores the organizational evolution of megaproject management teams through a longitudinal retrospective case study of the Hong Kong–Zhuhai–Macao Bridge (HZMB) project. The organizational evolution is examined in terms of management objectives, management content, and organizational structure. The organizational evolution of the HZMB project management team exhibits stage differentiation with the coexistence of turbulence and stability. Changes in the external environment are the driving force for organizational evolution, whereas a flexible organizational strategy is critical in promoting this evolution. Basing on the HZMB case study, this paper summarizes six critical measures that facilitate the organizational evolution of megaproject management teams. Our findings add value to megaproject management theory and provide a better understanding of the dynamics and complexity of megaproject organizational management.

关键词： megaproject organizational evolution whole lifecycle management project management team

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TCGA whole-transcriptome sequencing data reveals significantly dysregulated genes and signaling pathways

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《医学前沿（英文）》 2015年第9卷第3期页码 322-330 doi: 10.1007/s11684-015-0408-9

摘要：

This study systematically evaluates the TCGA whole-transcriptome sequencing data of hepatocellular carcinoma (HCC) by comparing the global gene expression profiles between tumors and their corresponding non-tumorous liver tissue. Based on the differential gene expression analysis, we identified a number of novel dysregulated genes, in addition to those previously reported. Top-listing upregulated (CENPF and FOXM1) and downregulated (CLEC4G, CRHBP, and CLEC1B) genes were successfully validated using qPCR on our cohort of 65 pairs of human HCCs. Further examination for the mechanistic overview by subjecting significantly upregulated and downregulated genes to gene set enrichment analysis showed that different cellular pathways were involved. This study provides useful information on the transcriptomic landscape and molecular mechanism of hepatocarcinogenesis for development of new biomarkers and further in-depth characterization.

关键词： TCGA whole-transcriptome sequencing HCC liver cancer

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Strategic Thinking on Shallow-water Oil and Gas Exploration in Offshore China

Ke-qiang Wu,Jian-yong Xu,Bo Kang

《工程管理前沿（英文）》 2016年第3卷第4期页码 349-355 doi: 10.15302/J-FEM-2016056

摘要： The oil and gas exploration in offshore China has made great achievements, and as the important areas of offshore exploration, the shallow waters contribute most of the oil and gas reserves and production. However, the available area for exploration is diminishing and the cost of exploration is rising. The field changes and ever increasing difficulties of exploration bring new challenges technically and economically. Therefore, for effective exploration of shallow-water oil and gas in offshore China, it is necessary to center on the general requirements of becoming a powerful marine country and the ideas of value exploration, initiate key research projects in the fields of China’s offshore stratigraphic-lithologic reserves, Paleogene reserves, buried-hill reserves, high temperature and pressure reserves and hydrocarbon-rich sags, and form geological theories and explorative technologies of large and medium size oil and gas field in offshore China. The measures regarding the challenges include: (1) Innovating theoretical understanding and optimizing new exploration fields, (2) developing technical capabilities and improving the success rate of exploration, and (3) enhancing management level and deepening value exploration.

关键词： offshore China shallow-water oil and gas exploration strategic thinking challenge countermeasure

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Analysis of the genomic landscape of primary central nervous system lymphoma using whole-genome sequencing

《医学前沿（英文）》页码 889-906 doi: 10.1007/s11684-023-0994-x

摘要： Primary central nervous system lymphoma (PCNSL) is an uncommon non-Hodgkin’s lymphoma with poor prognosis. This study aimed to depict the genetic landscape of Chinese PCNSLs. Whole-genome sequencing was performed on 68 newly diagnosed Chinese PCNSL samples, whose genomic characteristics and clinicopathologic features were also analyzed. Structural variations were identified in all patients with a mean of 349, which did not significantly influence prognosis. Copy loss occurred in all samples, while gains were detected in 77.9% of the samples. The high level of copy number variations was significantly associated with poor progression-free survival (PFS) and overall survival (OS). A total of 263 genes mutated in coding regions were identified, including 6 newly discovered genes (ROBO2, KMT2C, CXCR4, MYOM2, BCLAF1, and NRXN3) detected in ≥ 10% of the cases. CD79B mutation was significantly associated with lower PFS, TMSB4X mutation and high expression of TMSB4X protein was associated with lower OS. A prognostic risk scoring system was also established for PCNSL, which included Karnofsky performance status and six mutated genes (BRD4, EBF1, BTG1, CCND3, STAG2, and TMSB4X). Collectively, this study comprehensively reveals the genomic landscape of newly diagnosed Chinese PCNSLs, thereby enriching the present understanding of the genetic mechanisms of PCNSL.

关键词： primary central nervous system lymphoma whole-genome sequencing TMSB4X copy number variation gene mutation

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Cell surface protein engineering for high-performance whole-cell catalysts

Hajime Nakatani,Katsutoshi Hori

《化学科学与工程前沿（英文）》 2017年第11卷第1期页码 46-57 doi: 10.1007/s11705-017-1609-3

摘要： Cell surface protein engineering facilitated by accumulation of information on genome and protein structure involves heterologous production and modification of cell surface proteins using genetic engineering, and is important for the development of high-performance whole-cell catalysts. In this field, cell surface display is a major technology by exposing target proteins, such as enzymes, on the cell surface using a carrier protein. The target proteins are fused to the carrier proteins that transport and tether them to the cell surface, as well as to a secretion signal. This paper reviews cell surface display systems for prokaryotic and eukaryotic cells from the perspective of carrier proteins, which determine the number of displayed molecules, and the localization, size, and direction ( or terminal anchoring) of the passengers. We also discuss advanced methods for displaying multiple enzymes and a new method for the immobilization of whole-cell catalysts using adhesive surface proteins.

关键词： cell surface engineering surface display whole-cell catalysts bioprocess

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Efficient acetoin production from pyruvate by engineered whole-cell biocatalysis

《化学科学与工程前沿（英文）》 2023年第17卷第4期页码 425-436 doi: 10.1007/s11705-022-2229-0

摘要： Acetoin is an important platform chemical, which has a wide range of applications in many industries. Halomonas bluephagenesis, a chassis for next generation of industrial biotechnology, has advantages of fast growth and high tolerance to organic acid salts and alkaline environment. Here, α-acetolactate synthase and α-acetolactate decarboxylase from Bacillus subtilis 168 were co-expressed in H. bluephagenesis to produce acetoin from pyruvate. After reaction condition optimization and further increase of α-acetolactate decarboxylase expression, acetoin production and yield were significantly enhanced to 223.4 mmol·L^–1 and 0.491 mol·mol^–1 from 125.4 mmol·L^–1 and 0.333 mol·mol^–1, respectively. Finally, the highest titer of 974.3 mmol·L^–1 (85.84 g·L^–1) of acetoin was accumulated from 2143.4 mmol·L^–1 (188.6 g·L^–1) of pyruvic acid within 8 h in fed-batch bioconversion under optimal reaction conditions. Moreover, the reusability of the cell catalysis was also tested, and the result illustrated that the whole-cell catalysis obtained 433.3, 440.2, 379.0, 442.8 and 339.4 mmol·L^–1 (38.2, 38.8, 33.4, 39.0 and 29.9 g·L^–1) acetoin in five repeated cycles under the same conditions. This work therefore provided an efficient H. bluephagenesis whole-cell catalysis with a broad development prospect in biosynthesis of acetoin.

关键词： acetoin pyruvate α-acetolactate synthetase α-acetolactate decarboxylase Halomonas bluephagenesis whole-cell biocatalysis

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Calling systematic thinking in addressing water security issues

Jining CHEN, Pengfei DU

《环境科学与工程前沿（英文）》 2013年第7卷第5期页码 625-625 doi: 10.1007/s11783-013-0564-6

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From systematicness to complexity: Fundamental thinking of mega-project management

Zhaohan SHENG, Han LIN

《工程管理前沿（英文）》 2018年第5卷第1期页码 125-127 doi: 10.15302/J-FEM-2018001

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Transforming bacterial disease surveillance and investigation using whole-genome sequence to probe the

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《医学前沿（英文）》 2018年第12卷第1期页码 23-33 doi: 10.1007/s11684-017-0607-7

摘要：

Two decades have passed since the first bacterial whole-genome sequencing, which provides new opportunity for microbial genome. Consequently, considerable genetic diversity encoded by bacterial genomes and among the strains in the same species has been revealed. In recent years, genome sequencing techniques and bioinformatics have developed rapidly, which has resulted in transformation and expedited the application of strategy and methodology for bacterial genome comparison used in dissection of infectious disease epidemics. Bacterial whole-genome sequencing and bioinformatic computing allow genotyping to satisfy the requirements of epidemiological study in disease control. In this review, we outline the significance and summarize the roles of bacterial genome sequencing in the context of bacterial disease control and prevention. We discuss the applications of bacterial genome sequencing in outbreak detection, source tracing, transmission mode discovery, and new epidemic clone identification. Wide applications of genome sequencing and data sharing in infectious disease surveillance networks will considerably promote outbreak detection and early warning to prevent the dissemination of bacterial diseases.

关键词： genome sequencing genomic epidemiology bacteria surveillance infectious diseases